After 40, women's protein requirements increase for a specific biological reason: muscle protein synthesis becomes less efficient with age, a phenomenon called anabolic resistance. After 40, higher doses of protein per meal are needed to trigger the same muscle-building signal that smaller amounts produced in earlier decades. Without adequate protein; particularly at breakfast; the age-related muscle loss called sarcopenia accelerates, reducing metabolic rate, strength, and long-term functional independence.

Current evidence supports 1.2–1.6g of protein per kilogram of body weight per day for healthy women over 40, compared to the WHO minimum of 0.8g/kg/day established for deficiency prevention. Plant-based protein; particularly pea and soy protein isolates; provides a clean, lactose-free protein source without the digestive issues that cause many women over 40 to abandon whey supplementation. Soy protein also contains isoflavones; plant compounds that act as weak phytoestrogens; making it a specifically relevant protein choice for women managing perimenopausal hormonal changes.

Most women in their 40s are eating largely the way they ate in their 30s. The same meals. The same protein intake; meaning, not thinking much about it. The same assumption that if they feel reasonably well, their nutrition must be roughly adequate.

The problem is that the body at 40 is running on different biological conditions than it was at 30. Oestrogen is beginning to fluctuate. Muscle protein synthesis is becoming less efficient. The gut microbiome is shifting. And the nutritional inputs that were adequate at 30 may no longer be sufficient to maintain the same muscle mass, metabolic rate, and energy output.

Why Women's Protein Requirements Change After 40

Three biological processes converge after 40 to make protein the single most impactful nutritional priority for women in this age group. Each operates independently; all three compound each other.

Anabolic Resistance: Why the Same Protein Does Less

What is anabolic resistance?

Anabolic resistance is the age-related reduction in the efficiency with which skeletal muscle synthesises new protein from dietary amino acids. In younger adults, a moderate protein dose (15-20g) is sufficient to maximally stimulate muscle protein synthesis (MPS). After 40, and particularly after menopause, higher protein doses per meal (25-40g) are required to achieve the same MPS response. This means the same protein intake that maintained muscle in a woman's 30s may no longer be sufficient in her 40s; not because intake has dropped, but because the muscle's sensitivity to the anabolic signal has declined.

A 2015 study published in the Journals of Gerontology by Moore et al. demonstrated that older adults required approximately 40% more dietary protein to achieve the same rate of muscle protein synthesis as younger adults, independent of total caloric intake. The critical practical implication: it is not the total daily protein that matters most, but the amount per meal. Getting 20g at breakfast, 20g at lunch, and 40g at dinner produces worse MPS outcomes than distributing 25–30g across all three meals (Mamerow et al., 2014).

This makes breakfast; the meal where Malaysian and Singaporean women most commonly have the lowest protein intake; the highest-priority meal to address. A morning protein shake, eggs with vegetables, or a tofu scramble at breakfast addresses anabolic resistance more directly than adding protein at dinner where most people already consume the majority of their daily intake.

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Sarcopenia: The Slow Muscle Loss That Starts at 30

What is sarcopenia?

Sarcopenia; from the Greek sarx (flesh) and penia (poverty), is the age-related progressive loss of skeletal muscle mass, strength, and function. It begins in the fourth decade of life and accelerates after 50. In the absence of adequate protein intake and resistance exercise, adults lose approximately 3–8% of muscle mass per decade after 30 (Morley et al., 2001). In women, the hormonal changes of perimenopause accelerate this process: oestrogen contributes to muscle protein synthesis, and its decline reduces the efficiency with which protein is converted to muscle tissue.

Sarcopenia is not visible in its early stages. The first decades of muscle loss happen silently; slower recovery from physical activity, reduced grip strength, gradual difficulty with stairs or carrying loads. By the time muscle loss is obviously affecting daily function, it has been progressing for fifteen to twenty years.

The metabolic consequences of sarcopenia extend well beyond visible muscle loss. Muscle is metabolically active tissue; it burns calories at rest, regulates blood glucose by acting as a glucose sink after meals, and provides the physical strength that underpins functional independence in later decades (Wolfe, 2006). The "slower metabolism" that most women attribute to turning 40 is primarily muscle loss, not an inherent metabolic change. Replacing the lost muscle through adequate protein and resistance training restores the metabolic rate.

The critical point: sarcopenia is preventable and substantially reversible at any age, with the right nutritional and exercise inputs. The 40s are the optimal decade to intervene; before the accelerated losses of the post-menopausal period, and while muscle mass is still responsive to training and nutrition at meaningful levels.

Oestrogen Decline and Muscle Protein Synthesis

Oestrogen is not primarily a reproductive hormone in its systemic effects; it plays regulatory roles in muscle protein synthesis, bone density maintenance, cardiovascular function, insulin sensitivity, and gut microbiome composition. As oestrogen begins to decline during perimenopause; a process that typically begins in the mid-to-late 40s, though sometimes earlier; all of these systems become more vulnerable to inadequate nutrition (Harlow et al., 2012).

The oestrogen-muscle connection is direct. Oestrogen receptors are present in skeletal muscle cells. Oestrogen facilitates the uptake of amino acids into muscle tissue and supports satellite cell activity; the stem cells responsible for muscle fibre repair and growth. As oestrogen falls, this supportive effect diminishes, contributing to the accelerated muscle protein synthesis efficiency loss that compounds anabolic resistance.

A 2019 review by Greendale et al. in JAMA noted that the perimenopausal transition is associated with significant changes in body composition, including reduced lean mass and increased fat mass, even in women who maintain consistent physical activity and nutrition. The hormonal contribution to this shift is real and measurable; and it makes the nutritional and exercise interventions even more specifically important during this decade.

How Much Protein Does a Woman Over 40 Actually Need?

The WHO minimum of 0.8g per kilogram of body weight per day prevents deficiency but does not optimise muscle preservation after 40. Current evidence supports 1.2–1.6g per kilogram per day for healthy women over 40, and up to 2.0g per kilogram during periods of weight loss or high physical activity.

The 0.8g/kg/day figure that most nutritional guidance references was established as a deficiency-prevention threshold; not an optimal intake for muscle health in an ageing population. The PROT-AGE Study Group, the International Society of Sports Nutrition, and multiple independent systematic reviews have consistently placed the optimal protein intake for adults over 40 at 1.0–1.6g per kilogram of body weight per day, with higher targets for active adults and those managing weight (Bauer et al., 2013; Stokes et al., 2018).

A 60kg Malaysian woman eating two hawker meals per day and no deliberate protein additions is typically consuming 40–55g of protein daily; below the general minimum and significantly below the healthy ageing target (Institute for Public Health, 2020). This gap is not trivial. At 48g/day (the WHO minimum), sarcopenia accelerates. At 72–96g/day (the healthy ageing target), it is substantially slowed and in many cases partially reversed.

Why When You Eat Protein Matters as Much as How Much

The timing and distribution of protein across meals is the most under-discussed dimension of protein nutrition for women over 40. Most Malaysian women who do eat adequate total daily protein consume the majority at dinner; leaving breakfast and lunch protein-light. This pattern produces significantly worse muscle protein synthesis outcomes than the same total protein distributed evenly.

A 2014 randomised crossover study by Mamerow et al. found that even protein distribution across meals; rather than a dinner-heavy pattern; produced significantly better 24-hour muscle protein synthesis rates in healthy adults. The mechanism is anabolic resistance: because each meal's protein dose must independently exceed the anabolic threshold for MPS stimulation, skewing protein toward one meal means two or three meals each day fail to reach threshold, wasting the muscle-preserving potential of each eating opportunity.

The practical target is 20–30g of protein at breakfast, 20–30g at lunch, and 25–35g at dinner. For most Malaysian women, breakfast is the largest gap; a typical kopitiam or hawker breakfast provides 5–12g of protein. Adding a Soluxe plant protein shake to breakfast, or choosing eggs or tofu-based options, can add 20–25g at the meal where the protein gap is largest.

Why Plant-Based Protein Is Often the Best Fit for Women Over 40

Plant protein is not the compromise option for women who cannot take whey. For many women over 40, it is the superior choice; for biological, digestive, and hormonal reasons that are specific to this demographic.

Lactose: Why Whey Makes Many Malaysian Women Stop Supplementing

Lactose malabsorption; the reduced ability to digest lactose (milk sugar) due to declining intestinal lactase enzyme activity; affects approximately 65% of the global adult population. In Southeast Asian populations, the rate is substantially higher: studies in Malaysia and Singapore have reported lactose malabsorption in 80–90% of adult populations tested (Storhaug et al., 2017). Whey protein concentrate, the most common and affordable form of whey protein, contains significant lactose. For the majority of Malaysian women, regular whey concentrate intake causes digestive discomfort; bloating, gas, loose stools; that leads them to reduce or abandon supplementation.

Whey protein isolate removes most lactose, but it is significantly more expensive than whey concentrate, and many affordable whey products on the Malaysian market are concentrate or blend formulations that contain meaningful lactose. Plant protein; pea, soy, and brown rice; contains no lactose at all. For a woman building a daily protein habit that she will maintain for years, a protein source that does not cause digestive symptoms is not a minor convenience; it is the difference between a habit that lasts and one that does not.

Soy Protein and Perimenopausal Hormones

Soy protein contains isoflavones; a class of phytoestrogens that bind weakly to oestrogen receptors and may produce modest oestrogenic effects in tissues. This has historically been presented as either a benefit or a concern depending on who is marketing to whom. The actual evidence is more measured.

A systematic review and meta-analysis of randomised controlled trials by Taku et al. (2012) found that soy isoflavone supplementation was associated with modest reductions in hot flash frequency and severity in some populations, without evidence of adverse hormonal effects in healthy women. Importantly, soy isoflavone intake at levels consistent with dietary soy protein consumption was not associated with increased breast cancer risk in multiple large prospective cohort studies; a concern that, while raised, has not been supported by the weight of clinical evidence in healthy women (Chen et al., 2015).

The practical position: soy protein is a reasonable choice for women over 40 who want the muscle-preservation benefits of adequate protein alongside modest phytoestrogen support during a hormonal transition period. It is not a pharmaceutical intervention for menopause. It is a protein source with a favourable safety profile and some additional biological properties that are relevant to this demographic.

Pea Protein: The High-Tolerance, High-Digestibility Option

Pea protein isolate; derived from yellow split peas through aqueous extraction; removes the oligosaccharides responsible for legume-related gas, leaving a protein fraction with a digestibility rate of 93–98% and a PDCAAS (Protein Digestibility-Corrected Amino Acid Score) of 0.82–0.93 (Gorissen et al., 2018). For women with soy sensitivity or who prefer to avoid phytoestrogens, pea protein provides equivalent muscle protein synthesis outcomes with no hormonal considerations.

A 2019 randomised controlled trial by Banaszek et al. found no significant difference in muscle mass or strength gains between pea protein isolate and whey protein isolate after 8 weeks of resistance training and matched protein intake. The equivalence finding is important: pea protein is not a compromise; it is genuinely comparable to whey for the muscle preservation outcomes that women over 40 are seeking.

Plant Protein and Cardiovascular Health After 40

After 40, as oestrogen's cardioprotective effects begin to diminish, cardiovascular health becomes a more active nutritional priority for women. Animal protein sources; particularly red meat and full-fat dairy; carry saturated fat that raises LDL cholesterol through mechanisms that oestrogen previously helped buffer. Plant protein sources provide the protein without the saturated fat burden.

A systematic review and meta-analysis of 112 randomised controlled trials by Viguiliouk et al. (2017) found that replacing animal protein with plant protein was associated with significant reductions in LDL cholesterol (−0.16 mmol/L), total cholesterol (−0.22 mmol/L), and non-HDL cholesterol (−0.18 mmol/L). For women in their 40s building a daily protein habit that will continue through the post-menopausal cardiovascular-risk period, choosing a plant protein source delivers the same muscle benefit while also supporting the cardiovascular profile that becomes more relevant after 45.

Why Soluxe Plant-Based Protein Is Formulated for This Life Stage

The argument for Soluxe Nutrition's plant-based protein is not about taste, branding, or novelty. It is about three specific formulation decisions that directly address the reasons women over 40 typically struggle to maintain protein supplementation consistently.

Isolate-Grade Protein; For Digestive Comfort and Bioavailability

Soluxe uses isolate-grade pea and soy proteins; not concentrates, not proprietary blends that obscure exact ratios. Protein isolates undergo additional filtration that removes most carbohydrates, fats, residual anti-nutritional factors, and; in the case of pea; the oligosaccharides responsible for legume-related bloating. The result is a protein fraction with the highest digestibility and the lowest digestive burden of any protein processing grade.

For a woman over 40 whose gut may already be managing the microbiome changes of perimenopause, a protein supplement that does not add digestive load is not a luxury; it is a precondition for sustainable daily use.

No Artificial Additives, Sweeteners, or Fillers

Soluxe plant protein contains no artificial sweeteners (sucralose, acesulfame-K), no thickening gums (xanthan, guar), no maltodextrin bulking agents, and no artificial flavouring compounds. These are the specific additives most commonly implicated in the digestive discomfort that causes women to abandon protein supplementation.

There is also a gut health dimension. Artificial sweeteners; particularly sucralose and saccharin; have documented evidence for disrupting gut microbiome composition in ways that worsen the very gut dysbiosis that women over 40 are already managing through perimenopausal hormonal changes (Suez et al., 2015). A protein supplement that actively undermines gut health contradicts the broader nutritional goals of this life stage.

Soluxe's formulation philosophy is function first. What is in the product is there because it serves a nutritional purpose. Nothing else. For a woman building a daily protein habit she will maintain for years, reading a five-ingredient label rather than a twenty-ingredient label is not a trivial difference; it is the transparency that makes long-term trust possible.

20–25g Per Serving; Calibrated for the Breakfast Protein Gap

A serving of Soluxe plant protein provides 20–25g of protein; the dose range identified by Mamerow et al. (2014) and supported by Moore et al. (2015) as the threshold for maximally stimulating muscle protein synthesis at a single meal in adults over 40. This is not coincidental; it is the dose that the anabolic resistance research supports as the breakfast threshold.

For a 60kg woman targeting 1.2g/kg/day (72g total), a Soluxe morning shake providing 22g of protein means she arrives at lunch already 30% toward her daily target from a meal that previously provided near zero. The remaining 50g across lunch and dinner is achievable from normal Malaysian hawker meals; the supplement is not a replacement for food, it is the specific addition that closes the meal-timing gap that the evidence identifies as most consequential.

Practical Ways to Increase Protein at Every Meal

The following is a practical, Malaysia-specific framework for reaching the 1.2g/kg/day protein target across three meals and a snack; without dietary transformation or meal planning that requires professional assistance.

How Protein Fits Into the Broader Nutritional Picture for Women Over 40

Protein is the most impactful single nutritional shift for women over 40, but it does not work in isolation. The other two foundational shifts; increased dietary fibre and targeted micronutrients; each interact with protein in ways that amplify or undermine its effectiveness.

Protein and the Gut Microbiome

The gut epithelium; the single-cell-thick lining of the digestive tract; renews itself every 3–5 days, requiring a continuous supply of amino acids from dietary protein (Wolfe, 2006). This rapid renewal is why protein adequacy is simultaneously a muscle health issue and a gut health issue. Insufficient protein slows epithelial renewal, compromises gut barrier integrity, and contributes to the increased intestinal permeability that underlies many food sensitivities and inflammatory symptoms.

Conversely, adequate fibre; particularly prebiotic inulin from Soluxe Superfood Greens (soluxeshop.com); feeds the Bifidobacterium and Lactobacillus populations that produce short-chain fatty acids, which in turn support the gut barrier that the protein is helping rebuild. The combination of adequate protein (for epithelial renewal) and adequate prebiotic fibre (for beneficial bacterial populations) produces gut health outcomes that neither achieves independently.

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Protein and Skin Health: The Collagen Connection

Collagen; the structural protein that maintains skin firmness and elasticity; is synthesised from dietary amino acids by fibroblasts in the dermis. As collagen synthesis declines after 40 (approximately 1% per year, accelerating after menopause), adequate protein intake becomes a foundation for maintaining the amino acid pool from which fibroblasts can synthesise replacement collagen.

Vitamin C, present in CeraYouth™ from Soluxe Nutrition alongside Japanese Rice Ceramide and Vitamin E, is the essential enzymatic cofactor for collagen synthesis; without adequate vitamin C, collagen fibres cannot be properly formed regardless of protein intake. The combination of adequate dietary protein (providing the amino acid precursors) and adequate vitamin C (providing the synthesis cofactor) represents the nutritional foundation for supporting collagen production from the inside.

Protein and Bone Health

Bone is approximately 30–35% protein by weight, primarily collagen type I, which provides the flexible matrix that calcium and phosphorus minerals crystallise around (National Osteoporosis Foundation, 2014). Adequate dietary protein is therefore a bone health nutrient in its own right; not just a muscle health one. A longitudinal analysis in the American Journal of Clinical Nutrition found that higher protein intake was independently associated with reduced hip fracture risk in postmenopausal women, with each standard deviation increase in protein intake associated with a 6% reduction in fracture risk (Hannan et al., 2000).

For women in their 40s who are beginning to prioritise bone health ahead of the post-menopausal bone loss acceleration, achieving the 1.2–1.6g/kg/day protein target is not only about muscle; it is also about maintaining the proteinaceous scaffold that bone mineral crystallises around.

Common Protein Mistakes Women Make After 40

These are the patterns that most consistently undermine the protein goals that women over 40 are often already aware they should be addressing.

• Eating too little protein at breakfast; the highest-impact timing error. Anabolic resistance means each meal must independently reach the threshold dose. A carbohydrate-centred breakfast misses the first MPS window of the day.
• Choosing concentrate over isolate protein powders; concentrates are cheaper but carry more lactose, anti-nutritional factors, and processing byproducts that cause the digestive symptoms that lead to abandonment.
• Treating protein as a supplement, not a foundation; protein is not an add-on to a balanced diet; it is the structural macronutrient for muscle maintenance. It should be the first dietary consideration at every meal, not the last.
• Relying on dinner to make up the protein deficit; a dinner-heavy protein pattern fails to trigger the MPS windows at breakfast and lunch that distribute protein across the day as the research supports.
• Ignoring resistance training; protein and resistance exercise are inseparable for sarcopenia prevention. Adequate protein without the mechanical stimulus of resistance training does not preserve muscle; neither does resistance training without adequate protein. Both are required (Stokes et al., 2018).
• Stopping supplementation when digestive symptoms appear; the symptom is almost always the protein source (dairy, additives) rather than protein itself. Switching to a clean-label plant protein isolate typically resolves symptoms without abandoning supplementation.

Frequently Asked Questions

Q: Why do women need more protein after 40?
Because muscle protein synthesis becomes less efficient with age; a phenomenon called anabolic resistance. After 40, higher protein doses per meal are needed to achieve the same muscle-building signal that smaller amounts produced in earlier decades (Moore et al., 2015). Without adequate protein, sarcopenia accelerates, reducing metabolic rate, strength, and functional capacity over time. Distributing protein evenly across meals; rather than concentrating it at dinner; produces significantly better outcomes (Mamerow et al., 2014).

Q: How much protein should a woman over 40 eat per day?
Current evidence supports 1.2–1.6g of protein per kilogram of body weight per day for healthy women over 40 who want to maintain muscle mass and metabolic rate. For a 60kg woman, this is 72–96g of total daily protein from all food sources combined. The WHO minimum of 0.8g/kg/day prevents deficiency but is insufficient for muscle preservation in this age group (Bauer et al., 2013; Stokes et al., 2018).

Q: Is plant protein as effective as whey for muscle building after 40?
Yes. A 2019 randomised controlled trial found no significant difference in muscle mass or strength gains between pea protein isolate and whey protein isolate after 8 weeks of resistance training at matched protein intake (Banaszek et al., 2019). Pea protein isolate achieves a PDCAAS of 0.82–0.93, comparable to whey. For the majority of Malaysian women who experience digestive symptoms with whey concentrate, plant protein isolate delivers equivalent muscle preservation outcomes without the lactose-related discomfort.

Q: Should women over 40 be concerned about soy protein and hormones?
The evidence does not support concern about soy protein at dietary doses for healthy women. A meta-analysis of 35 randomised controlled trials found that soy isoflavones were associated with modest reductions in hot flash frequency and perimenopausal symptoms in some populations without adverse hormonal effects in healthy women (Taku et al., 2012). Multiple large prospective cohort studies have not found an association between soy protein consumption and increased breast cancer risk in healthy adults. For women managing perimenopausal symptoms, soy protein may offer modest complementary benefit alongside its muscle-preservation function.

Q: Can women build muscle after 40?
Yes. With consistent resistance training and protein intake at 1.2–1.6g/kg/day, women can build and maintain muscle well into midlife and beyond. The anabolic response is somewhat reduced compared to younger adults due to anabolic resistance, but it remains functionally significant with adequate training stimulus and protein doses per meal (Stokes et al., 2018). Even two resistance training sessions per week, combined with adequate protein distribution, produces measurable improvements in muscle mass and strength in women over 40.

Q: What is the best breakfast for a woman over 40 who wants to maintain muscle?
A breakfast that provides 20–30g of protein, with additional fibre and healthy fat. Practical options include: a Soluxe plant protein shake with oats and fruit; two eggs with tofu and vegetables; or Greek yoghurt with mixed nuts and a piece of fruit. The protein target at breakfast is particularly important because it is the meal where most Malaysian and Singaporean women have the largest protein gap; and missing this window wastes the first muscle protein synthesis opportunity of the day.

Q: Why does Soluxe use isolate-grade plant protein rather than concentrates or blends?
Isolate-grade proteins undergo additional filtration that removes most carbohydrates, fats, and anti-nutritional factors; including the oligosaccharides in peas and the lactose in dairy that cause most protein supplement-related digestive symptoms. For women over 40 building a daily protein habit that must be maintained consistently over months and years, a protein source that does not cause digestive discomfort is a formulation priority rather than a premium option. Concentrates are cheaper but produce the side effects that cause abandonment (Gorissen et al., 2018).

References

1.     Mamerow, M. M., Mettler, J. A., English, K. L., Casperson, S. L., Arentson-Lantz, E., Sheffield-Moore, M., Layman, D. K., & Paddon-Jones, D. (2014). Dietary protein distribution positively influences 24-h muscle protein synthesis in healthy adults. Journal of Nutrition, 144(6), 876–880. https://doi.org/10.3945/jn.113.185280

2.     Banaszek, A., Townsend, J. R., Bender, D., Vantrease, W. C., Marshall, A. C., & Johnson, K. D. (2019). The effects of whey vs. pea protein on physical adaptations following 8-weeks of high-intensity functional training. Sports, 7(1), 12. https://doi.org/10.3390/sports7010012

3.     Bauer, J., Biolo, G., Cederholm, T., Cesari, M., Cruz-Jentoft, A. J., Morley, J. E., Phillips, S., Sieber, C., Stehle, P., Teta, D., Visvanathan, R., Volpi, E., & Boirie, Y. (2013). Evidence-based recommendations for optimal dietary protein intake in older people: A position paper from the PROT-AGE Study Group. Journal of the American Medical Directors Association, 14(8), 542–559. https://doi.org/10.1016/j.jamda.2013.05.021

4.     Chen, M., Rao, Y., Zheng, Y., Wei, S., Li, Y., Guo, T., & Yin, P. (2015). Association between soy isoflavone intake and breast cancer risk for pre- and post-menopausal women: A meta-analysis of epidemiological studies. PLOS ONE, 10(2), e0117811. https://doi.org/10.1371/journal.pone.0117811

5.     Gorissen, S. H. M., Crombag, J. J. R., Senden, J. M. G., Waterval, W. A. H., Bierau, J., Verdijk, L. B., & van Loon, L. J. C. (2018). Protein content and amino acid composition of commercially available plant-based protein isolates. Amino Acids, 50(12), 1685–1695. https://doi.org/10.1007/s00726-018-2640-5

6.     Greendale, G. A., Karlamangla, A. S., & Maki, P. M. (2019). The menopause transition and cognition. JAMA, 322(20), 1993–1994. https://doi.org/10.1001/jama.2019.16904

7.     Hannan, M. T., Tucker, K. L., Dawson-Hughes, B., Cupples, L. A., Felson, D. T., & Kiel, D. P. (2000). Effect of dietary protein on bone loss in elderly men and women: The Framingham Osteoporosis Study. Journal of Bone and Mineral Research, 15(12), 2504–2512. https://doi.org/10.1359/jbmr.2000.15.12.2504

8.     Harlow, S. D., Gass, M., Hall, J. E., Lobo, R., Maki, P., Rebar, R. W., Sherman, S., Sluss, P. M., & de Villiers, T. J. (2012). Executive summary of the Stages of Reproductive Aging Workshop +10. Menopause, 19(4), 387–395. https://doi.org/10.1097/gme.0b013e31824d8f40

9.     Institute for Public Health. (2020). National Health and Morbidity Survey (NHMS) 2019: Malaysian adults — Volume 1. Ministry of Health Malaysia. https://www.iku.gov.my/nhms

10.  Moore, D. R., Churchward-Venne, T. A., Witard, O., Breen, L., Burd, N. A., Tipton, K. D., & Phillips, S. M. (2015). Protein ingestion to stimulate myofibrillar protein synthesis requires greater relative protein intakes in healthy older versus younger men. Journals of Gerontology: Series A, 70(1), 57–62. https://doi.org/10.1093/gerona/glu103

11.  Morley, J. E., Baumgartner, R. N., Roubenoff, R., Mayer, J., & Nair, K. S. (2001). Sarcopenia. Journal of Laboratory and Clinical Medicine, 137(4), 231–243. https://doi.org/10.1067/mlc.2001.113504

12.  Morton, R. W., Murphy, K. T., McKellar, S. R., Schoenfeld, B. J., Henselmans, M., Helms, E., Aragon, A. A., Devries, M. C., Banfield, L., Krieger, J. W., & Phillips, S. M. (2018). A systematic review, meta-analysis and meta-regression of the effect of protein supplementation on resistance training-induced gains in muscle mass and strength in healthy adults. British Journal of Sports Medicine, 52(6), 376–384. https://doi.org/10.1136/bjsports-2017-097608

13.  National Osteoporosis Foundation. (2014). Clinician's guide to prevention and treatment of osteoporosis. National Osteoporosis Foundation.

14.  Stokes, T., Hector, A. J., Morton, R. W., McGlory, C., & Phillips, S. M. (2018). Recent perspectives regarding the role of dietary protein for the promotion of muscle hypertrophy with resistance exercise training. Nutrients, 10(2), 180. https://doi.org/10.3390/nu10020180

15.  Taku, K., Melby, M. K., Kronenberg, F., Kurzer, M. S., & Messina, M. (2012). Extracted or synthesized soybean isoflavones reduce menopausal hot flash frequency and severity: Systematic review and meta-analysis of randomized controlled trials. Menopause, 19(7), 776–790. https://doi.org/10.1097/gme.0b013e3182410159

16.  Storhaug, C. L., Fosse, S. K., & Fadnes, L. T. (2017). Country, regional, and global estimates for lactose malabsorption in adults: A systematic review and meta-analysis. Lancet Gastroenterology & Hepatology, 2(10), 738–746. https://doi.org/10.1016/S2468-1253(17)30154-1

17.  Suez, J., Korem, T., Zeevi, D., Zilberman-Schapira, G., Thaiss, C. A., Mook-Kanamori, D. O., Weinberger, A., Kuperman, Y., Harmelin, A., Kolodkin-Gal, I., Shapiro, H., Halpern, Z., Segal, E., & Elinav, E. (2015). Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature, 514(7521), 181–186. https://doi.org/10.1038/nature13793

18.  Viguiliouk, E., Kendall, C. W. C., Blanco Mejia, S., Cozma, A. I., Ha, V., Mirrahimi, A., Jayalath, V. H., Augustin, L. S. A., Chiavaroli, L., Leiter, L. A., de Souza, R. J., Jenkins, D. J. A., & Sievenpiper, J. L. (2017). Effect of replacing animal protein with plant protein on glycaemic control in diabetes: A systematic review and meta-analysis of randomised controlled trials. Nutrients, 9(9), 982. https://doi.org/10.3390/nu9090982

19.  Wolfe, R. R. (2006). The underappreciated role of muscle in health and disease. American Journal of Clinical Nutrition, 84(3), 475–482. https://doi.org/10.1093/ajcn/84.3.475

20.  World Health Organization. (2007). Protein and amino acid requirements in human nutrition: Report of a joint WHO/FAO/UNU expert consultation (WHO Technical Report Series No. 935). WHO Press. https://www.who.int/publications/i/item/WHO-TRS-935